ICC/IF analysis of NIH-3T3 cells using GTX03181 ARF1 antibody [GT1269]. Blue : DAPI for nuclear staining Dilution : 1:100
![Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03181/GTX03181_4000001472_20210625_WB_2_w_23053123_914.webp "Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03181/GTX03181_4000001472_20210625_WB_w_23053123_788.webp "Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody [GT1269] (GTX03181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![WB analysis of various samples using GTX03181 ARF1 antibody [GT1269]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03181/GTX03181_58_WB_w_23053123_133.webp "WB analysis of various samples using GTX03181 ARF1 antibody [GT1269]. Dilution : 1:1000 Loading : 25microg per lane")
ICC/IF analysis of NIH-3T3 cells using GTX03181 ARF1 antibody [GT1269]. Blue : DAPI for nuclear staining Dilution : 1:100
ARF1 antibody [GT1269]
GTX03181
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetARF1
Overview
- SupplierGeneTex
- Product NameARF1 antibody [GT1269]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1269
- Concentration0.3 mg/ml
- ConjugateUnconjugated
- Gene ID375
- Target nameARF1
- Target descriptionADP ribosylation factor 1
- Target synonymsADP-ribosylation factor 1; PVNH8
- HostRabbit
- IsotypeIgG
- Protein IDP84077
- Protein NameADP-ribosylation factor 1
- Scientific DescriptionADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203