IHC-P analysis of human colon carcinoma tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human liver tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_1138_IHC-P_w_23060620_316.webp "IHC-P analysis of human liver tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![IHC-P analysis of human tonsil tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_1139_IHC-P_w_23060620_977.webp "IHC-P analysis of human tonsil tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![FACS analysis of 3T3 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_148_FACS_w_23060620_179.webp "FACS analysis of 3T3 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of Hela cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_149_FACS_w_23060620_267.webp "FACS analysis of Hela cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_150_FACS_w_23060620_651.webp "FACS analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9] compared to an isotype control (blue). Dilution : 1:80 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![WB analysis of canine heart extract using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_1601_WB_w_23060620_109.webp "WB analysis of canine heart extract using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9].")
![ICC/IF analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_480_ICC-IF_w_23060620_926.webp "ICC/IF analysis of MCF-7 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_481_ICC-IF_w_23060620_876.webp "ICC/IF analysis of HeLa cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22806/GTX22806_482_ICC-IF_w_23060620_832.webp "ICC/IF analysis of U251 cells using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human colon carcinoma tissue using GTX22806 ARF1/ARF3/ARF5/ARF6 antibody [1D9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
ARF1/ARF3/ARF5/ARF6 antibody [1D9]
GTX22806
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Hamster, Human, Mouse, Primate, Rat
Overview
- SupplierGeneTex
- Product NameARF1/ARF3/ARF5/ARF6 antibody [1D9]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects ADP-ribosylation factor1 (Arf1), Arf3, Arf5 and Arf6 and about ten-fold less well with Arf4.
- Application Supplier NoteWB: 1:500. ICC/IF: 1:500. IHC-P: 1:100. FACS: 1/20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1d9
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityBovine, Canine, Hamster, Human, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203