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Arp2 antibody [N1C3]

GTX103311
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetACTR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Arp2 antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.41 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10097
  • Target name
    ACTR2
  • Target description
    actin related protein 2
  • Target synonyms
    actin-like protein 2; actin-related protein 2; ARP2; ARP2 actin related protein 2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61160
  • Protein Name
    Actin-related protein 2
  • Scientific Description
    The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A perfusion-based three-dimensional cell culture system to model alveolar rhabdomyosarcoma pathological features.
    Read more
  • The WAVE complex associates with sites of saddle membrane curvature. Pipathsouk A et al., 2021 Aug 2, J Cell Biol
    Read more
  • Cell confinement reveals a branched-actin independent circuit for neutrophil polarity. Graziano BR et al., 2019 Oct, PLoS Biol
    Read more
  • In vivo quantitative proteomics of somatosensory cortical synapses shows which protein levels are modulated by sensory deprivation. Butko MT et al., 2013 Feb 19, Proc Natl Acad Sci U S A
    Read more
  • hnRNP Q regulates Cdc42-mediated neuronal morphogenesis. Chen HH et al., 2012 Jun, Mol Cell Biol
    Read more

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Figure 1. Western blot analysis of Arp2/ACTR2 using anti-Arp2/ACTR2 antibody (A03898-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse HEPA1-6 wholel cell lysates, Lane 4: mouse SP2/0 whole cell lysates, Lane 5: monkey COS-7 whole cell lysates, Lane 6: human U-87MG whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human PC-3 whole cell lysates, Lane 9: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Arp2/ACTR2 antigen affinity purified polyclonal antibody (Catalog # A03898-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Arp2/ACTR2 at approximately 45KD. The expected band size for Arp2/ACTR2 is at 45KD.
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