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Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody (TA378271) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody (TA378271) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.
Western blot analysis of extracts of A-431 and HeLa cells, using Phospho-MAP3K5-S1033 antibody (TA378271) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA.

ASK1 (MAP3K5) Rabbit Polyclonal Antibody

Research Use Only
TA378271
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMAP3K5
€ 477,00
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ASK1 (MAP3K5) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4217
  • Target name
    MAP3K5
  • Target description
    mitogen-activated protein kinase kinase kinase 5
  • Target synonyms
    apoptosis signal-regulating kinase 1; ASK1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Phospho-MAP3K5-S1033 Rabbit polyclonal Antibody
  • Reactivity
    Human
  • UNSPSC
    12352203