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Western blot analysis of extracts of Jurkat cells, using Phospho-MAP3K5-S967 antibody (TA378275) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Western blot analysis of extracts of Jurkat cells, using Phospho-MAP3K5-S967 antibody (TA378275) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.
Western blot analysis of extracts of Jurkat cells, using Phospho-MAP3K5-S967 antibody (TA378275) at 1:1000 dilution.Jurkat cells were treated by CIP(20uL/400ul) at 37°C for 1 hour.Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Enhanced Kit . - Exposure time: 180s.

ASK1 (MAP3K5) Rabbit Polyclonal Antibody

Research Use Only
TA378275
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetMAP3K5
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ASK1 (MAP3K5) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4217
  • Target name
    MAP3K5
  • Target description
    mitogen-activated protein kinase kinase kinase 5
  • Target synonyms
    apoptosis signal-regulating kinase 1; ASK1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99683
  • Protein Name
    Mitogen-activated protein kinase kinase kinase 5
  • Scientific Description
    Phospho-MAP3K5-S967 Rabbit polyclonal Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203