Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ASK1 (Ser966) Polyclonal Antibody (bs-3007R)at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ASK1 (Ser966) Polyclonal Antibody (bs-3007R)at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
ASK1 (Ser966) Polyclonal Antibody
BS-3007R
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Equine, Human, Mouse, Rat
TargetMAP3K5
Overview
- SupplierBioss Antibodies
- Product NameASK1 (Ser966) Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID4217
- Target nameMAP3K5
- Target descriptionmitogen-activated protein kinase kinase kinase 5
- Target synonymsapoptosis signal-regulating kinase 1; ASK1; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; MEKK5; mitogen-activated protein kinase kinase kinase 5
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- ReactivityCanine, Chicken, Equine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203