Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody(GTX40848). Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.
Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody(GTX40848). Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.
Asparaginase antibody (HRP)
GTX40848
Overview
- SupplierGeneTex
- Product NameAsparaginase antibody (HRP)
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:5000. ELISA: 1:10000-1:40000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration10 mg/ml
- ConjugateHRP
- HostRabbit
- IsotypeIgG
- Scientific DescriptionAsparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity.E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme.
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- An urchin-like helical polypeptide-asparaginase conjugate with mitigated immunogenicity. Hu Y et al., 2021 Jan, BiomaterialsRead more