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Product Information: KinaseSTARTM Akt Activity Assay Kit: Akt Activity Assay Kit utilizing an Akt specific Antibody to Immunoprecipitate Akt from Cell lysate. Simple & Convenient. 40 Assays. Background: Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate. Phosphorylation of the GSK-3alpha can be analyzed by Western blot analysis using the phospho-GSK-3alpha specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected. Summary: Detection method- Western blot analysis using anti-Phospho-GSK-3alpha (Ser 21) antibody at 1:1000 dilutions Species reactivity- Mammalian Applications- The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3alpha as substrate.
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