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Alkaline Phosphatase (ALP) Assay Kit (Colorimetric)

Research Use Only
LS-K307
LifeSpan BioSciences
Product group Assays
Price on request
Packing Size
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Alkaline Phosphatase (ALP) Assay Kit (Colorimetric)
  • Delivery Days Customer
    14
  • Applications Supplier
    Spectrophotometry (visible) For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate.
  • Certification
    Research Use Only
  • Scientific Description
    Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. Simple, direct and automation-ready procedures for measuring ALP activity in serum are becoming popular in Research and Drug Discovery. This Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved Method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
  • Storage Instruction
    2°C to 8°C or -20°C
  • UNSPSC
    41116133