Bovine Fibronectin ELISA Kit

Catalog number: MBS2885120_48wells
Brand: MyBioSource
Packing: 48 wells
Price: € 527.00
Expected delivery time: 21 days

Product specifications for - Bovine Fibronectin ELISA Kit

Product group: Assays
Category: ELISA / EIA
Species: Bovine
Target: FN1
Target description: fibronectin 1
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: -20-¦C
Scientific information: 
Scientific info: Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of bovine fibronectin,FN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Gene ID: 280794
Swiss prot ID: P07589
Additional information: 
Synonyms: MBS2885120; cumulus cell-specific fibronectin 1 transcript; embryo-specific fibronectin 1 transcript; fibronectin; fibronectin ED-A- region; fibronectin variable region; FN