FOXO Reporter Kit (PI3K/AKT Pathway)

Catalog number: 60643
Brand: BPS Bioscience
Packing: 500 reactions
Price: € 747.00
Expected delivery time: 7 days

Product specifications for - FOXO Reporter Kit (PI3K/AKT Pathway)

Product group: Assays
Category: Cell based assays
Subcategory: Reporter
Application note: Monitor PI3K/AKT signaling pathway activity and FOXO transcriptional activity.
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41106513
Form supplied: assay kit
Storage instructions: -20-¦C
Scientific information: 
Scientific info: The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
Additional information: 
Synonyms: 60643; BPS Bioscience
Essaghir, A., et al. (2009). J. Biol. Chem. 284(16):10334-10342 Read more
Hennessy B.T., et al. (2005). Nat. Rev. Drug Discov. 4(12):988-1004. Application Reference(s): FOXO responses to Porphyromonas gingivalis in epithelial cells (2015) Read more