FOXO Reporter Kit (PI3K/AKT Pathway)
60643
Product group Assays
Overview
- SupplierBPS Bioscience
- Product NameFOXO Reporter Kit (PI3K/AKT Pathway)
- Delivery Days Customer7
- CertificationResearch Use Only
- Scientific DescriptionThe FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity.
- Storage Instruction-20°C
- UNSPSC41116133
References
- Essaghir, A., et al. (2009). J. Biol. Chem. 284(16):10334-10342
- Hennessy B.T., et al. (2005). Nat. Rev. Drug Discov. 4(12):988-1004. Application Reference(s): FOXO responses to Porphyromonas gingivalis in epithelial cells (2015)