Human Collagenase I ELISA Kit

Catalog number: MBS2505413_48stripwells
Brand: MyBioSource
Packing: 48 strip Wells
Other sizes: Other sizes available, please contact us.
Price: € 504.00
Expected delivery time: 21 days
Typical Testing Data/Standard Curve (for reference only)

Product specifications for - Human Collagenase I ELISA Kit

Product group: Assays
Category: ELISA / EIA
Species: Human
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: Store at 4-¦C.
Scientific information: 
Scientific info: This ELISA kit applies to the invitro quantitative determination of HumanCollagenase Iconcentrations in serum, plasma and other biological fluids.Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Collagenase I. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Collagenase Iand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Collagenase I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Collagenase I.You can calculate the concentration of Collagenase Iin the samples by comparing the OD of the samples to the standard curve.
Gene ID: 2733197