PARP-1 [ARTD1] (E998K Mutant) (human) (rec.) Control

Catalog number: AG-40T-0015-C010
Brand: AdipoGen Life Sciences
Packing: 10 ug
Price: € 150.00
Expected delivery time: 7 days
Quantity:

Product specifications for - PARP-1 [ARTD1] (E998K Mutant) (human) (rec.) Control

Overview: 
Product group: Assays
Category: Standards / controls
Target: PARP1
Target description: poly(ADP-ribose) polymerase 1
Properties: 
Purity: >95% (SDS-PAGE)
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116135
Form supplied: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol.
Storage instructions: -20-¦C, -80-¦C
Scientific information: 
Scientific info: PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound. - Protein. Human full-length inactive mutant E998K of PARP-1 is fused to a HA-tag and a His-tag. Source: Sf21 cells. Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride and 50mM imidazole, 0.2% NP-40 and 10% glycerol. Purity: >95% (SDS-PAGE). PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. PARP-1 (E998K mutant) is an inactive form of PARP-1 which can be used as a control compound.
Gene ID: 142
Swiss prot ID: P09874
Safety information: 
MSDS: MSDS
Hazard information: Non-hazardous
Additional information: 
Synonyms: AG-40T-0015-C010; AdipoGen Life Sciences; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase diphtheria toxin-like 1; ADP-ribosyltransferase NAD(+); ADPRT; ADPRT 1; ADPRT1; ARTD1; NAD(+) ADP-ribosyltransferase 1; pADPRT-1; PARP; PARP-1; poly (ADP-ribose) polymerase family, member 1; poly [ADP-ribose] polymerase 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; PPOL
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem. 276, 45588 (2001) Read more