Porcine Heparin ELISA Kit

Catalog number: MBS752573_48wells
Brand: MyBioSource
Packing: 48 wells
Price: € 579.00
Expected delivery time: 21 days

Product specifications for - Porcine Heparin ELISA Kit

Product group: Assays
Category: ELISA / EIA
Species: Porcine
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116158
Storage instructions: 2-¦C to 8-¦C
Scientific information: 
Scientific info: Intended Uses: This HEPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HEPA. This ELISA kit for research use only! Principle of the Assay: HEPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HEPA antibody and an HEPA-HRP conjugate. The assay sample and buffer are incubated together with HEPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HEPA concentration since HEPA from samples and HEPA-HRP conjugate compete for the anti-HEPA antibody binding site. Since the number of sites is limited, as more sites are occupied by HEPA from the sample, fewer sites are left to bind HEPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEPA concentration in each sample is interpolated from this standard curve.
Additional information: 
Synonyms: MBS752573