QuickTiter HBV Core Antigen ELISA Kit

Catalog number: VPK-150
Brand: Cell Biolabs
Packing: 96 assays
Other sizes: Other sizes available
Price: € 894.00
Expected delivery time: 7 days
Quantity:

Product specifications for - QuickTiter HBV Core Antigen ELISA Kit

Overview: 
Product group: Assays
Category: Cell based assays
Subcategory: Titer
Properties: 
Datasheet: Datasheet
  Research Use Only
UNSPSC: 41116133
Storage instructions: Upon receiving, aliquot and store recombinant HBVcAg Standard at -20-¦C and avoid freeze/thaw. Store all other components at 4-¦C.
Scientific information: 
Scientific info: An anti-HBVcAg monoclonal coating antibody is adsorbed onto a microtiter plate. HBV core antigen present in the sample or standard binds to the antibodies adsorbed on the plate, a FITC-conjugated mouse anti-HBVcAg antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated mouse anti-FITC antibody is added and binds to the FITC conjugated anti-HBVcAg. Following unbound HRP-conjugated mouse anti-FITC antibody is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of HBV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant HBV core antigen and sample concentration is then determined.
Safety information: 
MSDS: MSDS
Additional information: 
Synonyms: VPK-150; Cell Biolabs