Paraformaldehyde-fixed, paraffin embedded Human Colon carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF2 (Thr69/71) Polyclonal Antibody, Unconjugated (bs-3033R) at 1:200 overnight at 4°C, followed by secondary and DAB staining.
ATF2 (Thr69/71) Polyclonal Antibody
bs-3033R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetATF2
Overview
- SupplierBioss Antibodies
- Product NameATF2 (Thr69/71) Polyclonal Antibody
- Delivery Days Customer16
- Antibody SpecificityThis antibody is specific for ATF2 in when phosphorylated at both the Thr69 and Thr71 residues
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID1386
- Target nameATF2
- Target descriptionactivating transcription factor 2
- Target synonymsactivating transcription factor 2 splice variant ATF2-var2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB2; CREB-2; CRE-BP1; cyclic AMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; HB16; histone acetyltransferase ATF2; TREB7
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Protein IDP15336
- Protein NameCyclic AMP-dependent transcription factor ATF-2
- ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203