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ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] (GTX629815) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] (GTX629815) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
ATG12 antibody [GT166] detects ATG12 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 12% SDS-PAGE ATG12 antibody [GT166] (GTX629815) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

ATG12 antibody [GT166]

GTX629815
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, Other Application
Product group Antibodies
ReactivityHuman, Mouse
TargetATG12
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Overview

  • Supplier
    GeneTex
  • Product Name
    ATG12 antibody [GT166]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT166
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9140
  • Target name
    ATG12
  • Target description
    autophagy related 12
  • Target synonyms
    APG12, APG12L, FBR93, HAPG12, ubiquitin-like protein ATG12, APG12 autophagy 12-like, ATG12 autophagy related 12 homolog, Apg12 (autophagy, yeast) homolog
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDO94817
  • Protein Name
    Ubiquitin-like protein ATG12
  • Scientific Description
    Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Monaci S, Aldinucci C, Rossi D, et al. Hypoxia Shapes Autophagy in LPS-Activated Dendritic Cells. Front Immunol. 2020,11:573646. doi: 10.3389/fimmu.2020.573646
    Read this paper
  • Lin TY, Chan HH, Chen SH, et al. BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells. Autophagy. 2020,16(7):1296-1313. doi: 10.1080/15548627.2019.1671643
    Read this paper
  • Chang HP, Lu CC, Chiang JH, et al. Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling. Int J Oncol. 2018,52(5):1504-1514. doi: 10.3892/ijo.2018.4298
    Read this paper

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Figure 1. Western blot analysis of ATG12 using anti-ATG12 antibody (A00820-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human COLO 320 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG12 antigen affinity purified polyclonal antibody (Catalog # A00820-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATG12 at approximately 48 kDa. The expected band size for ATG12 is at 15 kDa.
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