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Western blot analysis of extracts of various cell lines, using ATP1A3 antibody (TA373807) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ATP1A3 antibody (TA373807) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ATP1A3 antibody (TA373807) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.

ATP1A3 Rabbit Polyclonal Antibody

TA373807
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetATP1A3
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ATP1A3 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID478
  • Target name
    ATP1A3
  • Target description
    ATPase Na+/K+ transporting subunit alpha 3
  • Target synonyms
    AHC2, ATP1A1, CAPOS, DEE99, DYT12, RDP, sodium/potassium-transporting ATPase subunit alpha-3, ATPase, Na+/K+ transporting, alpha 3 polypeptide, Na(+)/K(+) ATPase alpha(III) subunit, Na(+)/K(+) ATPase alpha-3 subunit, Na+, K+ activated adenosine triphosphatase alpha subunit, Na+/K+ ATPase 3, sodium pump subunit alpha-3, sodium-potassium ATPase catalytic subunit alpha-3, sodium-potassium-ATPase, alpha 3 polypeptide, sodium/potassium-transporting ATPase alpha-3 chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13637
  • Protein Name
    Sodium/potassium-transporting ATPase subunit alpha-3
  • Scientific Description
    ATP1A3 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ATP1A3 using anti-ATP1A3 antibody (A02278-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP1A3 antigen affinity purified polyclonal antibody (Catalog # A02278-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP1A3 at approximately 90 kDa. The expected band size for ATP1A3 is at 112 kDa.
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