IP analysis of THP-1 cell lysates using GTX25432 ATP5B antibody [4.3E8.D10]. GTX25432 was used as IP and IB antibody IP reaction : 5microl antibody / 500microg lysate Dilution : 1:1000
![WB analysis of rat neuronal/glial cell extract using GTX25432 ATP5B antibody [4.3E8.D10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX25432/GTX25432_1771_WB_w_23060722_838.webp "WB analysis of rat neuronal/glial cell extract using GTX25432 ATP5B antibody [4.3E8.D10].")
![ICC/IF analysis of 3T3 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (left) or with(right) an antibody. Green : ATPSynthase beta (ATP5B ) Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Dilution : 5 microg/ml for 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25432/GTX25432_637_ICC-IF_w_23060722_849.webp "ICC/IF analysis of 3T3 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (left) or with(right) an antibody. Green : ATPSynthase beta (ATP5B ) Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Dilution : 5 microg/ml for 1 hour at room temperature")
![ICC/IF analysis of HEK293T cells using GTX25432 ATP5B antibody [4.3E8.D10]. Red :ATPSynthase beta (ATP5B ) Blue : Nuclei Fixation : formaldehyde Permeabilization : 0.3% Triton X-100 Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25432/GTX25432_638_ICC-IF_w_23060722_216.webp "ICC/IF analysis of HEK293T cells using GTX25432 ATP5B antibody [4.3E8.D10]. Red :ATPSynthase beta (ATP5B ) Blue : Nuclei Fixation : formaldehyde Permeabilization : 0.3% Triton X-100 Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of A431 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25432/GTX25432_639_ICC-IF_w_23060722_905.webp "ICC/IF analysis of A431 cells using GTX25432 ATP5B antibody [4.3E8.D10]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of rat neuronal/glial cell culture using GTX25432 ATP5B antibody [4.3E8.D10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX25432/GTX25432_640_ICC-IF_w_23060722_976.webp "ICC/IF analysis of rat neuronal/glial cell culture using GTX25432 ATP5B antibody [4.3E8.D10].")
IP analysis of THP-1 cell lysates using GTX25432 ATP5B antibody [4.3E8.D10]. GTX25432 was used as IP and IB antibody IP reaction : 5microl antibody / 500microg lysate Dilution : 1:1000
ATP5B antibody [4.3E8.D10]
GTX25432
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAtp5f1b
Overview
- SupplierGeneTex
- Product NameATP5B antibody [4.3E8.D10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1-2 microg/ml. ICC/IF: 1:100-1:1000. IP: 2-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4.3E8.D10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID171374
- Target nameAtp5f1b
- Target descriptionATP synthase F1 subunit beta
- Target synonymsATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; Atp5b; mitochondrial ATP synthase beta subunit
- HostMouse
- IsotypeIgG1
- Scientific Descriptionbeta subunit of rat liver ATP synthase [RGD, Feb 2006]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203