Detection of endogenous Aurora-A: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal antibody AM20207PU-N (7F12) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Detection of endogenous Aurora-A: Whole cell extracts of vanadate treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The Immunoblot was probed with monoclonal antibody AM20207PU-N (7F12) (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF-7 Lane 6: MDA-MB231 Lane 7: T47D
Aurora A (AURKA) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]
AM20207PU-N
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetAURKA
Overview
- SupplierOriGene
- Product NameAurora A (AURKA) (N-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7F12]
- Delivery Days Customer14
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7F12
- Gene ID6790
- Target nameAURKA
- Target descriptionaurora kinase A
- Target synonymsAIK; ARK1; AURA; aurora 2; aurora kinase A; aurora/IPL1-like kinase; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; BTAK; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine/threonine protein kinase 15; serine/threonine-protein kinase 6; serine/threonine-protein kinase aurora-A; STK15; STK6; STK7
- HostMouse
- IsotypeIgG1
- Protein IDO14965
- Protein NameAurora kinase A
- Scientific DescriptionAurora A (AURKA) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 7F12, Purified
- ReactivityCanine, Human, Mouse, Rat
- UNSPSC12352203