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Azide-PEG3-FLAG

Research Use Only
ORB532286
Biorbyt
Estimated Purity≥95%
Product group Chemicals
Molecular WeightTheoretical MW: 1485.49 g/mol; Detected MW: 1484.57 g/mol
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Azide-PEG3-FLAG
  • Delivery Days Customer
    16
  • Certification
    Research Use Only
  • Estimated Purity
    ≥95%
  • Molecular Weight
    Theoretical MW: 1485.49 g/mol; Detected MW: 1484.57 g/mol
  • Scientific Description
    Azide-PEG 3-FLAG enables the FLAG-tag (DYKDDDDK) attachment to any terminal Alkyne-or Cyclooctyne (e.g. DBCO)-functionalized molecule via Cu(I)-catalyzed terminal Alkyne-Azide Click Chemistry (CuAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC), respectively. The resulting FLAG-tagged molecules can subsequently be de-tected by an anti-FLAG antibody that is either immobilized onto a matrix (for purification) or coupled with a fluorescent dye or reporter enzyme for direct or indirect detection, respectively. Molecule solu-bility and efficient FLAG-tag detection is ensured by the integrated PEG-linker. Azide-FLAG (without PEG-linker) has been successfully used for the detection of Azide-tagged glycoproteins in cell lysates (final concentration: 100 microM). This concentrations may serve as a starting point for individual assay set-up.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116134