Basic Cytokeratin AE3 Antibody (clone AE-3)

LifeSpan BioSciences

Basic Cytokeratin AE3 Antibody (clone AE-3)

23

Recognizes most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. Broadly reactive with almost all epithelial and their neoplasms. Species cross-reactivity: Rabbit, mouse, rat, bovine, chicken.

Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100 Detects distinct MWs of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ips lower then 6.0. These MWs are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37°C. Can also be used with unfixed frozen sections.. IHC, IHC-Fr, IHC-P, IP, WB (1:100) Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:100 Detects distinct MWs of 52, 54, 56, 58, 59, 64, and 65-67kD with all Ips lower then 6.0. These MWs are believed to correspond with keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Immunohistochemistry: 1:150-1:200 for use on formalin-fixed paraffin embedded tissues. Incubate for 30 minutes at 37°C. Can also be used with unfixed frozen sections.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

AE-3

Unconjugated

...

Mouse

IgG1

Bovine, Chicken, Mouse, Rabbit, Rat

-20°C,2°C to 8°C

12352203