Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Bax Monoclonal Antibody, Unconjugated (bsm-33279M) at 1:300 overnight at 4°C, followed by Goat Anti-Mouse IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
Bax Monoclonal Antibody
BSM-33279M
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat
TargetBAX
Overview
- SupplierBioss Antibodies
- Product NameBax Monoclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8H12
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsapoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
- HostMouse
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- ReactivityHuman, Mouse, Porcine, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Brg1 trans-activates endothelium-derived colony stimulating factor to promote calcium chloride induced abdominal aortic aneurysm in mice. Zhang X et al., 2018 Dec, J Mol Cell CardiolRead more
- Effects of Zearalenone Exposure on the TGF-beta1/Smad3 Signaling Pathway and the Expression of Proliferation or Apoptosis Related Genes of Post-Weaning Gilts. Zhou M et al., 2018 Jan 23, Toxins (Basel)Read more