Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (bs-0127M) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Bax Polyclonal Antibody
BS-0127M
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep, Other Species
TargetBAX
Overview
- SupplierBioss Antibodies
- Product NameBax Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsapoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
- HostMouse
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- ReactivityBovine, Canine, Human, Mouse, Porcine, Rat, Sheep, Other Species
- Storage Instruction-20°C
- UNSPSC12352203
References
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- Rotenone and 3-bromopyruvate toxicity impacts electrical and structural cardiac remodeling in rats. Zhan C et al., 2020 Jan, Toxicol LettRead more
- Effect of electroacupuncture at the ST36 and GB39 acupoints on apoptosis by regulating the p53 signaling pathway in adjuvant arthritis rats. Su C et al., 2019 Nov, Mol Med RepRead more
- Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. Kouam AF et al., 2017, Front PharmacolRead more
- A plasma mir-125a-5p as a novel biomarker for Kawasaki disease and induces apoptosis in HUVECs. Li Z et al., 2017, PLoS OneRead more
- A potential adjuvant chemotherapeutics, 18beta-glycyrrhetinic acid, inhibits renal tubular epithelial cells apoptosis via enhancing BMP-7 epigenetically through targeting HDAC2. Ma T et al., 2016 May 5, Sci RepRead more
- Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway. Qiao H et al., 2016, Oxid Med Cell LongevRead more