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ProductsBack/BCL10 / BCL-10 Antibody (clone OTI6A4)

BCL10 / BCL-10 Antibody (clone OTI6A4)

LS-C172542
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetBCL10
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCL10 / BCL-10 Antibody (clone OTI6A4)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Applications Supplier
    Flo (1:100), IF (1:100), WB (1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI6A4
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8915
  • Target name
    BCL10
  • Target description
    BCL10 immune signaling adaptor
  • Target synonyms
    B cell CLL/lymphoma 10; B-cell lymphoma/leukemia 10; CARD containing molecule enhancing NF-kB; CARD-containing apoptotic signaling protein; CARD-containing molecule enhancing NF-kappa-B; CARD-containing proapoptotic protein; CARMEN; caspase-recruiting domain-containing protein; cCARMEN; c-E10; CED-3/ICH-1 prodomain homologous E10-like regulator; cellular homolog of vCARMEN; cellular-E10; CIPER; CLAP; hCLAP; IMD37; mammalian CARD-containing adapter molecule E10; mE10
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Bcl10 using anti-Bcl10 antibody (PB9525). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl10 antigen affinity purified polyclonal antibody (Catalog # PB9525) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bcl10 at approximately 28 kDa. The expected band size for Bcl10 is at 26 kDa.
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