BDNF Human

Biosynth

BDNF Human

12

Research Use Only

97.0%

BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.DescriptionBrain-derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids (and an N-terminal Met) and having a total molecular mass of 27kDa.BDNF Human Recombinant is purified by proprietary chromatographic techniques.Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.FormulationThe protein was lyophilized without any additives.SolubilityIt is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.StabilityLyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).Please prevent freeze-thaw cycles.Biological ActivityThe ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2ug/ml.Protein contentBDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.

E.coli

12352202