beta 1 + 2 Adaptin antibody [100/1]

GeneTex

beta 1 + 2 Adaptin antibody [100/1]

10

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry

Research Use Only

Monoclonal

100/1

Mouse

IgG

Coated vesicle populations contain, besides clathrin, the adaptor complexes AP1 and AP2, also known as HAI (HA1) adaptor and HAII (HA2) adaptor or Assembly Protein 1 (AP1) and Assembly Protein 2 (AP2), respectively. Interest has focused on adaptors because they are known to mediate the interaction between clathrin and components of the membrane. Their location between the clathrin shell and the membrane, as revealed by cryoelectron microscopy, is consistent with such a function. In the cell, clathrin-adaptor interactions proceed in a very controlled and specific manner, leading to coated structures each containing only one type of adaptor. Clathrin-coated membranes at or originating from the plasma membrane contain the AP2 adaptor, while the AP1 adaptor appears to be largely restricted to clathrin-coated membranes of the trans-Golgi network. AP1 adaptor consists of four subunits termed beta 1 (formerly beta prime, 110 kDa), gamma (100 kDa), micro1 (47 kDa, and omega 1 (20 kDa). AP2 is made up of two 100-110 kDa polypeptides, termed alpha and beta 2 (formerly beta) subunits, micro2 (50 kDa), and s2 (17 kDa) subunit. Alpha and beta 2 subunits (alpha and beta 2 adaptins) behave in standard SDS-PAGE like 100-112 kDa polypeptides. Four alpha isoforms (alpha a1, alpha a2, alpha c1 and alpha c2) which are highly homologous in sequence have been described. ?1 and ?2 adaptins are highly homologous which suggests that the beta type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. The alpha and gamma adaptins share only 25% overall identity. The other adaptor subunits of the two adaptor complexes which appear to be unrelated, are believed to possess binding sites for different cargo molecules (receptors) and for proteins which might regulate the complex functions of the adaptors. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes.

Bovine, Human, Rat

2°C to 8°C,-20°C

12352203