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Beta-2-Microglobulin (B2M)(C21.48A1), CF405S conjugate, 0.1mg/mL [26628-22-8]

BNC040433
Biotium
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetB2M
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Overview

  • Supplier
    Biotium
  • Product Name
    Beta-2-Microglobulin (B2M)(C21.48A1), CF405S conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C21.48A1
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID567
  • Target name
    B2M
  • Target description
    beta-2-microglobulin
  • Target synonyms
    beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin; IMD43
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP61769
  • Protein Name
    Beta-2-microglobulin
  • Scientific Description
    Beta-2 microglobulin is a component of the class I major histocompatibility complex (MHC I) where it functions in antigen processing and presentation. Involved in the presentation of peptide antigens to the immune system. Human Beta-2 microglobulin is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This MAb is specific to human Beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. The detection of Beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 2. IHC analysis of Beta 2 Microglobulin using anti- Beta 2 Microglobulin antibody (A00456-1). Beta 2 Microglobulin was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti- Beta 2 Microglobulin Antibody (A00456-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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