WB analysis of tissue extracts (30 microg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2) using GTX24472 beta Arrestin 1 (phospho Ser412) antibody. Dilution : 0.5 microg/ml
WB analysis of tissue extracts (30 microg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2) using GTX24472 beta Arrestin 1 (phospho Ser412) antibody. Dilution : 0.5 microg/ml
beta Arrestin 1 (phospho Ser412) antibody
GTX24472
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetArrb1
Overview
- SupplierGeneTex
- Product Namebeta Arrestin 1 (phospho Ser412) antibody - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 0.1-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID25387
- Target nameArrb1
- Target descriptionarrestin, beta 1
- Target synonymsBARRES; beta-arrestin-1
- HostRabbit
- IsotypeIgG
- Protein IDP29066
- Protein NameBeta-arrestin-1
- Scientific DescriptionBeta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of G protein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine 412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203