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IHC-P analysis of human breast carcinoma tissue using GTX50256 beta Catenin (phospho Ser37) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human breast carcinoma tissue using GTX50256 beta Catenin (phospho Ser37) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide
IHC-P analysis of human breast carcinoma tissue using GTX50256 beta Catenin (phospho Ser37) antibody. Left : Primary antibody Right : Primary antibody pre-incubated with the antigen specific peptide

beta Catenin (phospho Ser37) antibody

GTX50256
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCTNNB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    beta Catenin (phospho Ser37) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:1000. IHC-P: 1:50-1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    CTNNB, EVR7, MRD19, NEDSDV, armadillo, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Scientific Description
    The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Xie B, Zhao L, Guo L, et al. Benzyl isothiocyanate suppresses development and metastasis of murine mammary carcinoma by regulating the Wnt/β‑catenin pathway. Mol Med Rep. 2019,20(2):1808-1818. doi: 10.3892/mmr.2019.10390
    Read this paper

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Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
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WB analysis of whole cell extracts (30 microg lysate) of HeLa (Lane 1), U-87 MG (Lane 2), COLO 205 (Lane 3), HCT 116 (lane 4), HT-29 (lane 5), MCF7 (lane 6), MDA-MB-231 (lane 7) and A549 (lane 8) using GTX80686 beta Catenin antibody [9F2]. Dilution : 1-3 microg/ml
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