More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/beta Catenin (phospho Ser675) antibody
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser675) antibody (GTX132611) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser675) antibody (GTX132611) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin (phospho Ser675) antibody (GTX132611) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

beta Catenin (phospho Ser675) antibody

GTX132611
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCTNNB1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    beta Catenin (phospho Ser675) antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.64 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1499
  • Target name
    CTNNB1
  • Target description
    catenin beta 1
  • Target synonyms
    CTNNB, EVR7, MRD19, NEDSDV, armadillo, catenin beta-1, catenin (cadherin-associated protein), beta 1, 88kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35222
  • Protein Name
    Catenin beta-1
  • Scientific Description
    Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the contact inhibition signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chang YX, Lin YF, Chen CL, et al. Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways. Cancers (Basel). 2020,12(12). doi: 10.3390/cancers12123865
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-beta catenin [15B8]Ab01655-1.1
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, Other Application
ReactivityBovine, Canine, Chicken, Human, Mouse, Rat
TargetCTNNB1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CTNNB1 Antibody144-11512
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTNNB1
  • SizePrice
Figure 1. Western blot analysis of CTNNB1 using anti-CTNNB1 antibody (A00004). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human T47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNB1 antigen affinity purified polyclonal antibody (Catalog # A00004) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTNNB1 at approximately 95 kDa. The expected band size for CTNNB1 is at 95 kDa.
Product group Antibodies
Boster Bio
Anti-beta Catenin/CTNNB1 Antibody Picoband(r)A00004-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCTNNB1
  • SizePrice
WB analysis of HeLa whole cell lysate using GTX00948 beta Catenin antibody [GT1186]. Dilution : 1:1000
Product group Antibodies
GeneTex
beta Catenin antibody [GT1186]GTX00948
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCTNNB1
  • SizePrice
beta Catenin antibody detects beta Catenin protein at membrane on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody (GTX101254) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Product group Antibodies
References
GeneTex
beta Catenin antibodyGTX101254
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetCTNNB1
  • SizePrice
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using beta Catenin antibody [N1N2-2], N-term (GTX101435) at 1:300 dilution.
Product group Antibodies
References
GeneTex
beta Catenin antibody [N1N2-2], N-termGTX101435
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityCanine, Feline, Human, Mouse, Rabbit, Rat, Zebra Fish
TargetCTNNB1
  • SizePrice
ChIP analysis of HCT116 cells using GTX10342 CTNNB1 antibody. Cells were prepared using the Farnham ChIP protocol. Use 2μg antibody GTX10342 CTNNB1 antibody for ChIP assay.
Product group Antibodies
GeneTex
beta Catenin antibody, InternalGTX10342
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation
ReactivityHuman
TargetCTNNB1
  • SizePrice
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE GTX110594 diluted at 1:1000
Product group Antibodies
GeneTex
beta Catenin antibodyGTX110594
ApplicationsWestern Blot
ReactivityHuman
TargetCTNNB1
  • SizePrice
The data was published in the journal PLoS Biol in 2018.
Product group Antibodies
References
GeneTex
beta Catenin (phospho Ser33/Ser37) antibody [BC-22]GTX11350
ApplicationsWestern Blot, ELISA
ReactivityChicken, Human, Mouse, Rat
TargetCTNNB1
  • SizePrice
beta Catenin antibody detects beta Catenin protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin stained by beta Catenin antibody (GTX632676) diluted at 1:1000.
Product group Antibodies
References
GeneTex
beta Catenin antibody [GT3171]GTX632676
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityCanine, Feline, Human, Mouse
TargetCTNNB1
  • SizePrice