Biotin-11-dATP

Biorbyt

Biotin-11-dATP

16

Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: lambdamax 280 nm, epsilon 12.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

1.0-1.1 mM

Liquid

Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

-20°C

12352200