Biotin-11-dCTP [136632-30-9]

Biorbyt

Biotin-11-dCTP [136632-30-9]

16

FISH; SNP-analysis; Nick-translation; TUNEL. Spectroscopic Propertie: lambdamax 294 nm, epsilon 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Research Use Only

1.0-1.1 mM

Liquid

Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/50% dCTP.

-20°C

12352200