BlasTaq HotStart DNA Polymerase

Applied Biological Materials

BlasTaq HotStart DNA Polymerase

9

Other Application

PCR and Proteins

Research Use Only

Eliminate non-specific amplification and primer-dimer formation! BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5-3 polymerase and 5-3 exonuclease activities, lacks 3-5 exonuclease activity, and produces 3-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. Product Features: No non-specific amplification or primer-dimers Improves yield of desired product Simplifies reaction setup

-20°C

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