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Illustration of the assay principle. BLM is incubated with the fluorescent DNA substrate in the presence of ATP. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.
Illustration of the assay principle. BLM is incubated with the fluorescent DNA substrate in the presence of ATP. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.
Illustration of the assay principle. BLM is incubated with the fluorescent DNA substrate in the presence of ATP. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.

BLM Helicase Activity Assay Kit

Research Use Only
82512
BPS Bioscience
Product group Assays
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More than 130 suppliers
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    BLM Helicase Activity Assay Kit
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • Scientific Description
    The BLM Helicase Activity Assay Kit is a fluorogenic assay designed for screening and profiling of BLM (Bloom syndrome protein) antagonists/inhibitors by monitoring their inhibitory effects on BLM-catalyzed DNA unwinding. The BLM Helicase Activity Assay Kit comes in a convenient 96-well format, with enough purified recombinant BLM (amino acids 630-1300), ATP, DNA substrate, assay buffer, and additives for 100 reactions. The DNA substrate is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. BLM unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. BLM activity, therefore, results in a proportional increase in fluorescence.
  • UNSPSC
    41116133