WB using Anti-Bmi1 antibody shows detection of a band ~37 kDa corresponding to human Bmi1 (arrowhead).Approximately 20 ug of a U2OS whole cell lysate (bone osteosarcoma) was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. The primary antibody diluted to 1:1,000 in PBS containing 1% nonfat dry milk. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MX for 45 min at room temperature.