Western blot showing detection of 0.1 microg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa). Lane 3: DbpA, MBP removed by TEV cleavage. Lane 4: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Western blot showing detection of 0.1 microg recombinant proteins in western blot. Lane 1: Molecular weight markers. Lane 2: MBP-DbpA fusion protein (arrow; expected MW: 60.9 kDa). Lane 3: DbpA, MBP removed by TEV cleavage. Lane 4: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Borrelia burgdorferi Dbp-A antibody
GTX48809
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBacteria
TargetdbpA
Overview
- SupplierGeneTex
- Product NameBorrelia burgdorferi Dbp-A antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1194347
- Target namedbpA
- Target descriptiondecorin-binding protein DbpA
- Target synonymsBB_A24; BB_RS05215; decorin-binding protein DbpA
- HostRabbit
- IsotypeIgG
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203