BrdU Antibody (clone BRD469 + BRD494 + BRD.3)

LifeSpan BioSciences

BrdU Antibody (clone BRD469 + BRD494 + BRD.3)

23

BrdU

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. The optimal dilution of the BrdU antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.. IF (0.5 - 1 µg/ml), IHC, IHC-P (0.5 - 1 µg/ml) It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. The optimal dilution of the BrdU antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.

ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

BRD469 + BRD494 + BRD.3

0.2 mg/ml

Unconjugated

Mouse

IgG1

2°C to 8°C

12352203