BUB1 antibody

Biorbyt

BUB1 antibody

16

This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 160 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. The use of HeLa whole cell lysates prepared using a RIPA buffer is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8 OR 100% methanol at -20° C. Permeabilize fixed cells with 0.5% Triton X-100.

ELISA: 1:5,000 - 1:20,000, IF: 1:500 - 1:2,000, IP: 1:200, WB: 1:500 - 1:2,000 ELISA, IF, IP, WB

Research Use Only

Monoclonal

14H5

1 mg/ml

Unconjugated

Liquid

BUB1

BUB1 mitotic checkpoint serine/threonine kinase

BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1A; BUB1L; budding uninhibited by benzimidazoles 1 homolog; hBUB1; mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase

Mouse

IgG1

Mitotic checkpoint serine/threonine-protein kinase BUB1

BUB1 antibody

Human

-20°C,2°C to 8°C

12352203