ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2]. Left : Untreated cells Right : Cells stimulated with membrane deplorization buffer for 5 hours. Green : Primary antibody Red : GFAP antibody Blue : DAPI
![ICC/IF analysis of HeLa cells using GTX60996 c-Fos antibody [2H2]. Left : Serum-starved HeLa cells Right : Serum-starved and then stimulated with 20% FBS for 2 hours Green : Primary antibody Red : Vimentin antibody Blue : DAPI Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60996/GTX60996_20231130_ICCIF_23112922_697.webp "ICC/IF analysis of HeLa cells using GTX60996 c-Fos antibody [2H2]. Left : Serum-starved HeLa cells Right : Serum-starved and then stimulated with 20% FBS for 2 hours Green : Primary antibody Red : Vimentin antibody Blue : DAPI Dilution : 1:1000")
![IHC-P analysis of mouse hippocampus tissue using using GTX60996 c-Fos antibody [2H2] Antigen retrieval : Citrate buffer Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60996/GTX60996_20231130_IHC-P_1_23112922_783.webp "IHC-P analysis of mouse hippocampus tissue using using GTX60996 c-Fos antibody [2H2] Antigen retrieval : Citrate buffer Dilution : 1:1000")
![IHC-P analysis of rat hippocampus tissue using using GTX60996 c-Fos antibody [2H2] Antigen retrieval : Citrate buffer Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60996/GTX60996_20231130_IHC-P_23112922_526.webp "IHC-P analysis of rat hippocampus tissue using using GTX60996 c-Fos antibody [2H2] Antigen retrieval : Citrate buffer Dilution : 1:1000")
![WB analysis of various samples using GTX60996 c-Fos antibody [2H2]. Lane 1: Protein marker (red) Lane 2: HeLa cells in serum free media Lane 3: HeLa cells stimulated with 20% FBS for 2hrs after 36hrs in serum free media. Lane 4: Rat cortical neurons Lane 5: Rat cortical neurons treated with membrane depolarization buffer for 5hrs. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX60996/GTX60996_20231130_WB_23112922_835.webp "WB analysis of various samples using GTX60996 c-Fos antibody [2H2]. Lane 1: Protein marker (red) Lane 2: HeLa cells in serum free media Lane 3: HeLa cells stimulated with 20% FBS for 2hrs after 36hrs in serum free media. Lane 4: Rat cortical neurons Lane 5: Rat cortical neurons treated with membrane depolarization buffer for 5hrs. Dilution : 1:1000")
![c-Fos antibody [2H2] detects c-Fos protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. c-Fos stained by c-Fos antibody [2H2] (GTX60996) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX60996/GTX60996_822304774_20240202_IHC-P_multiple_M_24021917_107.webp "c-Fos antibody [2H2] detects c-Fos protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. c-Fos stained by c-Fos antibody [2H2] (GTX60996) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2]. Left : Untreated cells Right : Cells stimulated with membrane deplorization buffer for 5 hours. Green : Primary antibody Red : GFAP antibody Blue : DAPI
c-Fos antibody [2H2]
GTX60996
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOS
Overview
- SupplierGeneTex
- Product Namec-Fos antibody [2H2]
- Delivery Days Customer9
- Application Supplier NoteWestern blots: 1:1,000-2,000, ICC/IF or IHC-Fr: 1:1,000
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2H2
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2353
- Target nameFOS
- Target descriptionFos proto-oncogene, AP-1 transcription factor subunit
- Target synonymsactivator protein 1; AP-1; cellular oncogene c-fos; C-FOS; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); FBJ murine osteosarcoma viral oncogene homolog; Fos proto-oncogene, AP-1 trancription factor subunit; G0/G1 switch regulatory protein 7; p55; proto-oncogene c-Fos
- HostMouse
- IsotypeIgG1
- Protein IDP01100
- Protein NameProto-oncogene c-Fos
- Scientific DescriptionThe Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- NMDA Receptors Control Activity Hierarchy in Neural Network: Loss of Control in Hierarchy Leads to Learning Impairments, Dissociation, and Psychosis.Read more
- Effects of Motivational Downshifts on Specific Pavlovian-Instrumental Transfer in Rats.Read more
- The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice. Chang CH et al., 2019 Jul 30, Cell RepRead more
- Eye-Drops for Activation of DREADDs.Read more