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ProductsBack/c-Fos antibody [2H2]
ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2]. Left : Untreated cells Right : Cells stimulated with membrane deplorization buffer for 5 hours. Green : Primary antibody Red : GFAP antibody Blue : DAPI
ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2]. Left : Untreated cells Right : Cells stimulated with membrane deplorization buffer for 5 hours. Green : Primary antibody Red : GFAP antibody Blue : DAPI
ICC/IF analysis of rat brain neural cultured cells using GTX60996 c-Fos antibody [2H2]. Left : Untreated cells Right : Cells stimulated with membrane deplorization buffer for 5 hours. Green : Primary antibody Red : GFAP antibody Blue : DAPI

c-Fos antibody [2H2]

GTX60996
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOS
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Overview

  • Supplier
    GeneTex
  • Product Name
    c-Fos antibody [2H2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Western blots: 1:1,000-2,000, ICC/IF or IHC-Fr: 1:1,000
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2H2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01100
  • Protein Name
    Protein c-Fos
  • Scientific Description
    The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zhou Y, Wang JL, Qiu L, et al. NMDA Receptors Control Activity Hierarchy in Neural Network: Loss of Control in Hierarchy Leads to Learning Impairments, Dissociation, and Psychosis. bioRxiv. 2024,:pii: 2023.01.06.523038. doi: 10.1101/2023.01.06.523038.
    Read this paper
  • Sommer S, Münster A, Fehrentz JA, et al. Effects of Motivational Downshifts on Specific Pavlovian-Instrumental Transfer in Rats. Int J Neuropsychopharmacol. 2022,25(3):173-184. doi: 10.1093/ijnp/pyab075
    Read this paper
  • Chang CH, Gean PW. The Ventral Hippocampus Controls Stress-Provoked Impulsive Aggression through the Ventromedial Hypothalamus in Post-Weaning Social Isolation Mice. Cell Rep. 2019,28(5):1195-1205.e3. doi: 10.1016/j.celrep.2019.07.005
    Read this paper
  • Keenan WT, Fernandez DC, Shumway LJ, et al. Eye-Drops for Activation of DREADDs. Front Neural Circuits. 2017,11:93. doi: 10.3389/fncir.2017.00093
    Read this paper

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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