IHC-P analysis of human skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of mouse skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_1180_IHC-P_w_23060620_224.webp "IHC-P analysis of mouse skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates using GTX22864 CACNA2D1 antibody [20A]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_1617_WB_w_23060620_175.webp "WB analysis of 25 ug of U87-MG (lane 1) and mouse brain (lane 2) cell lysates using GTX22864 CACNA2D1 antibody [20A]. Dilution : 1:1000")
![FACS analysis of C6 cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_169_FACS_w_23060620_515.webp "FACS analysis of C6 cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of Neuro-2a cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_170_FACS_w_23060620_530.webp "FACS analysis of Neuro-2a cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![FACS analysis of SH-SY5Y cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_171_FACS_w_23060620_858.webp "FACS analysis of SH-SY5Y cells using GTX22864 CACNA2D1 antibody [20A] compared to an isotype control (blue). Dilution : 1:100 for 60 min at room temperature Fixation : 2% paraformaldehyde")
![ICC/IF analysis of PC-12 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_525_ICC-IF_w_23060620_184.webp "ICC/IF analysis of PC-12 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_526_ICC-IF_w_23060620_729.webp "ICC/IF analysis of HeLa cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22864/GTX22864_527_ICC-IF_w_23060620_226.webp "ICC/IF analysis of U251 cells using GTX22864 CACNA2D1 antibody [20A]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human skeletal muscle tissue using GTX22864 CACNA2D1 antibody [20A]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
CACNA2D1 antibody [20A]
GTX22864
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFish, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
TargetCACNA2D1
Overview
- SupplierGeneTex
- Product NameCACNA2D1 antibody [20A]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:250. IHC-P: 1:500. IHC-Fr: 1:500. FACS: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID20A
- ConjugateUnconjugated
- Gene ID100009105
- Target nameCACNA2D1
- Target descriptioncalcium voltage-gated channel auxiliary subunit alpha2delta 1
- Target synonymscalcium channel, voltage-dependent, alpha 2/delta subunit 1; dihydropryridine-sensitive calcium channel alpha-2 subunit; voltage-dependent calcium channel subunit alpha-2/delta-1; voltage-gated calcium channel subunit alpha-2/delta-1
- HostMouse
- IsotypeIgG2a
- Protein IDP13806
- Protein NameVoltage-dependent calcium channel subunit alpha-2/delta-1
- ReactivityFish, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Okada T et al., 2021 Mar, Sci AdvRead more