WB analysis of purified calmodulin protein using GTX25494 Calmodulin antibody [6D4].
![ICC/IF analysis of HeLa cells using GTX25494 Calmodulin antibody [6D4]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25494/GTX25494_681_ICC-IF_w_23060722_406.webp "ICC/IF analysis of HeLa cells using GTX25494 Calmodulin antibody [6D4]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature")
WB analysis of purified calmodulin protein using GTX25494 Calmodulin antibody [6D4].
Calmodulin antibody [6D4]
GTX25494
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityBacteria, Bovine, Chicken, Human, Mouse, Rat
Overview
- SupplierGeneTex
- Product NameCalmodulin antibody [6D4]
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK). Addition of EGTA to buffers completely inhibits antibody staining.
- Application Supplier NoteWB: 1:2,000. ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6D4
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific DescriptionCalmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations.
- ReactivityBacteria, Bovine, Chicken, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203