IHC-P analysis of human kidney tissue using GTX23589 Calpain 1 antibody [9A4H8D3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human tonsil tissue using GTX23589 Calpain 1 antibody [9A4H8D3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_1240_IHC-P_w_23060722_320.webp "IHC-P analysis of human tonsil tissue using GTX23589 Calpain 1 antibody [9A4H8D3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![WB analysis of 15 ug of HeLa cell lysate (lane 2), 15 ug of A431 cell lysate (lane 3) using GTX23589 Calpain 1 antibody [9A4H8D3]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_1702_WB_w_23060722_853.webp "WB analysis of 15 ug of HeLa cell lysate (lane 2), 15 ug of A431 cell lysate (lane 3) using GTX23589 Calpain 1 antibody [9A4H8D3]. Dilution : 1:500")
![WB analysis of membrane enriched extracts (30 microg lysate) of HeLa (Lane 1), A-431 (Lane 2), MOLT-4 (Lane 3), SK-OV-3 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) using GTX23589 Calpain 1 antibody [9A4H8D3]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_1703_WB_w_23060722_993.webp "WB analysis of membrane enriched extracts (30 microg lysate) of HeLa (Lane 1), A-431 (Lane 2), MOLT-4 (Lane 3), SK-OV-3 (Lane 4), LNCaP (Lane 5) and PC-3 (Lane 6) using GTX23589 Calpain 1 antibody [9A4H8D3]. Dilution : 2 microg/ml")
![ICC/IF analysis of MCF-7 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_609_ICC-IF_w_23060722_435.webp "ICC/IF analysis of MCF-7 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_610_ICC-IF_w_23060722_818.webp "ICC/IF analysis of HeLa cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX23589/GTX23589_611_ICC-IF_w_23060722_300.webp "ICC/IF analysis of U251 cells using GTX23589 Calpain 1 antibody [9A4H8D3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4oC")
IHC-P analysis of human kidney tissue using GTX23589 Calpain 1 antibody [9A4H8D3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
Calpain 1 antibody [9A4H8D3]
GTX23589
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Fish, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast, Other Species
TargetCAPN1
Overview
- SupplierGeneTex
- Product NameCalpain 1 antibody [9A4H8D3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin and it recognizes an epitope between amino acids 465-520 (domain III) of human mu-calpain.
- Application Supplier NoteWB: 2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID9A4H8D3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID281661
- Target nameCAPN1
- Target descriptioncalpain 1
- Target synonymscalcium-activated neutral proteinase 1; calpain 1, (mu/I) large subunit; calpain mu-type; calpain-1 catalytic subunit; calpain-1 large subunit; CANP 1; CANP1; micromolar calcium-activated neutral protease 1; micromolar-calpain; mu-calpain; muCANP
- HostMouse
- IsotypeIgG1
- Protein IDQ27970
- Protein NameCalpain-1 catalytic subunit
- ReactivityBovine, Fish, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203