IHC-P analysis of human heart tissue using GTX22824 Calsequestrin antibody [VIIID12]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human skeletal muscle tissue using GTX22824 Calsequestrin antibody [VIIID12]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX22824/GTX22824_1161_IHC-P_w_23060620_203.webp "IHC-P analysis of human skeletal muscle tissue using GTX22824 Calsequestrin antibody [VIIID12]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![WB analysis of canine skeletal muscle extract using GTX22824 Calsequestrin antibody [VIIID12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22824/GTX22824_1610_WB_w_23060620_945.webp "WB analysis of canine skeletal muscle extract using GTX22824 Calsequestrin antibody [VIIID12].")
![ICC/IF analysis of MCF-7 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22824/GTX22824_508_ICC-IF_w_23060620_314.webp "ICC/IF analysis of MCF-7 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22824/GTX22824_509_ICC-IF_w_23060620_311.webp "ICC/IF analysis of HeLa cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of U251 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22824/GTX22824_510_ICC-IF_w_23060620_590.webp "ICC/IF analysis of U251 cells using GTX22824 Calsequestrin antibody [VIIID12]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human heart tissue using GTX22824 Calsequestrin antibody [VIIID12]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Calsequestrin antibody [VIIID12]
GTX22824
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Human, Mouse, Porcine, Rabbit, Rodent, Rat
TargetCASQ1
Overview
- SupplierGeneTex
- Product NameCalsequestrin antibody [VIIID12]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:50-1:500. IHC-P: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDVIIID12
- ConjugateUnconjugated
- Gene ID100009095
- Target nameCASQ1
- Target descriptioncalsequestrin 1
- Target synonymsaspartactin; calsequestrin 1 (fast-twitch, skeletal muscle); calsequestrin-1; laminin-binding protein; skeletal muscle calsequestrin 1
- HostMouse
- IsotypeIgG2b
- Protein IDP07221
- Protein NameCalsequestrin-1
- ReactivityCanine, Chicken, Human, Mouse, Porcine, Rabbit, Rodent, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203