Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.
Immunofluorescent staining of heart sections from WT and MsrA-/- mice (a model of heart disease) treated with AngII and probed for oxidized (GTX36254) or total CaMKII and blue staining for nuclei.
CaMKII (oxidized) antibody
GTX36254
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse, Rat
Overview
- SupplierGeneTex
- Product NameCaMKII (oxidized) antibody
- Delivery Days Customer9
- Application Supplier NoteFor WB, use at a dilution of 1:1,000, For IF, use at a dilution of 1:50 with paraffin-embedded sections and 1:100 with frozen sections.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- HostRabbit
- IsotypeIgG
- Scientific DescriptionCalcium/calmodulin-dependent protein kinase II (CaMKII) couples increases in Ca2+ to fundamental responses in excitable cells including activation of ion channels, gene transcription and apoptosis. CaMKII is activated by angiotensin II-induced oxidation, which leads to apoptosis in cardiomyocytes. CaMKII activity is also enhanced by pro-oxidant conditions, and the oxidation of paired regulatory domain methionine residues sustains CaMKII activity in the absence of Ca2+/CaM.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
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- Zhao J, Yu L, Xue X, et al. Diminished α7 nicotinic acetylcholine receptor (α7nAChR) rescues amyloid-β induced atrial remodeling by oxi-CaMKII/MAPK/AP-1 axis-mediated mitochondrial oxidative stress. Redox Biol. 2023,59:102594. doi: 10.1016/j.redox.2022.102594Read this paper
- Luo X, Yu W, Liu Z, et al. Ageing Increases Cardiac Electrical Remodelling in Rats and Mice via NOX4/ROS/CaMKII-Mediated Calcium Signalling. Oxid Med Cell Longev. 2022,2022:8538296. doi: 10.1155/2022/8538296Read this paper
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- Joseph LC, Reyes MV, Homan EA, et al. The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet. Sci Rep. 2021,11(1):17808. doi: 10.1038/s41598-021-97449-3Read this paper
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- Pradhan K, Geng S, Zhang Y, et al. TRAM-Related TLR4 Pathway Antagonized by IRAK-M Mediates the Expression of Adhesion/Coactivating Molecules on Low-Grade Inflammatory Monocytes. J Immunol. 2021,206(12):2980-2988. doi: 10.4049/jimmunol.2000978Read this paper