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Carbonic Anhydrase IX antibody [66.4.C2]

GTX01814
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCA9
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Overview

  • Supplier
    GeneTex
  • Product Name
    Carbonic Anhydrase IX antibody [66.4.C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:100-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    66.4.C2
  • Conjugate
    Unconjugated
  • Gene ID768
  • Target name
    CA9
  • Target description
    carbonic anhydrase 9
  • Target synonyms
    CAIX, MN, carbonic anhydrase 9, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase IX, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ16790
  • Protein Name
    Carbonic anhydrase 9
  • Scientific Description
    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jun 2014]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Ansari AN, Bobos M, Shih S, et al. Renal Cell Carcinoma Antigen Expression in Primary Cutaneous Endocrine Mucinous Carcinomas: A Case Series of 14 Patients and Review of the Literature. Am J Dermatopathol. 2019,41(8):571-577. doi: 10.1097/DAD.0000000000001370
    Read this paper
  • Fadare O, Desouki MM, Gwin K, et al. Clear Cell Renal Cell Carcinoma Metastatic to the Gynecologic Tract: A Clinicopathologic Analysis of 17 Cases. Int J Gynecol Pathol. 2018,37(6):525-535. doi: 10.1097/PGP.0000000000000466
    Read this paper
  • Braxton DR, Saxe D, Damjanov N, et al. Molecular and cytogenomic profiling of hepatic adenocarcinoma expressing inhibinA, a mimicker of neuroendocrine tumors: proposal to reclassify as "cholangioblastic variant of intrahepatic cholangiocarcinoma". Hum Pathol. 2017,62:232-241. doi: 10.1016/j.humpath.2017.02.001
    Read this paper
  • Lin WC, Chen JH, Westphalen A, et al. Primary Renal Rhabdomyosarcoma in an Adolescent With Tumor Thrombosis in the Inferior Vena Cava and Right Atrium: A Case Report and Review of the Literature. Medicine (Baltimore). 2016,95(21):e3771. doi: 10.1097/MD.0000000000003771
    Read this paper
  • Tajima S, Waki M, Doi W, et al. Acquired cystic disease-associated renal cell carcinoma with a focal sarcomatoid component: Report of a case showing more pronounced polysomy of chromosomes 3 and 16 in the sarcomatoid component. Pathol Int. 2015,65(2):89-94. doi: 10.1111/pin.12232
    Read this paper

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Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.
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