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ProductsBack/Carbonic Anhydrase IX (CA9) Rabbit Polyclonal Antibody
Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (TA374177) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.
Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (TA374177) at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit .<br/>Exposure time: 60s.
Western blot analysis of various lysates using Carbonic Anhydrase 9 (CA9/G250) Rabbit pAb (TA374177) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 60s.

Carbonic Anhydrase IX (CA9) Rabbit Polyclonal Antibody

TA374177
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCA9
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Carbonic Anhydrase IX (CA9) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID768
  • Target name
    CA9
  • Target description
    carbonic anhydrase 9
  • Target synonyms
    CAIX, MN, carbonic anhydrase 9, CA-IX, P54/58N, RCC-associated antigen G250, RCC-associated protein G250, carbonate dehydratase IX, carbonic anhydrase IX, carbonic dehydratase, membrane antigen MN, pMW1, renal cell carcinoma-associated antigen G250
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16790
  • Protein Name
    Carbonic anhydrase 9
  • Scientific Description
    Carbonic Anhydrase 9 (CA9/G250) Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Carbonic Anhydrase 9/CA9 using anti-Carbonic Anhydrase 9/CA9 antibody (A01083-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carbonic Anhydrase 9/CA9 antigen affinity purified polyclonal antibody (Catalog # A01083-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase 9/CA9 at approximately 54 kDa. The expected band size for Carbonic Anhydrase 9/CA9 is at 50 kDa.
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Lane 1: Rat Stomach lysates probed with Carbonic Anhydrase 9 Polyclonal Antibody, Unconjugated (bs-4029R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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