Western blot analysis of extracts from A431 cells untreated or treated with EGF(200ng/ml, 5min), using CARM1(Ab-228) antibody(, Line 1 and 2) and CARM1(Phospho-Ser228) antibody(, Line 3 and 4).
Western blot analysis of extracts from A431 cells untreated or treated with EGF(200ng/ml, 5min), using CARM1(Ab-228) antibody(, Line 1 and 2) and CARM1(Phospho-Ser228) antibody(, Line 3 and 4).
CARM1 (Ab-228) Antibody
CSB-PA021890
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCARM1
Overview
- SupplierCusabio
- Product NameCARM1 (Ab-228) Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID10498
- Target nameCARM1
- Target descriptioncoactivator associated arginine methyltransferase 1
- Target synonymshistone-arginine methyltransferase CARM1; PRMT4; protein arginine N-methyltransferase 4
- HostRabbit
- IsotypeIgG
- Protein IDQ86X55
- Protein NameHistone-arginine methyltransferase CARM1
- Scientific DescriptionMethylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg-17 and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at Arg-2142, which may loosen its interaction with NCOA2/GRIP1, and at Arg-580 and Arg-604 in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103(36): 13351
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C
- UNSPSC12352203