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ProductsBack/casein kinase IIalpha (D-7) Alexa Fluor(r) 647

casein kinase IIalpha (D-7) Alexa Fluor(r) 647

SC-514403AF647
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCSNK2A2
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    casein kinase IIalpha (D-7) Alexa Fluor(r) 647
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 647
  • Gene ID1459
  • Target name
    CSNK2A2
  • Target description
    casein kinase 2 alpha 2
  • Target synonyms
    CK2A2, CK2alpha', CSNK2A1, casein kinase II subunit alpha', CK II alpha', casein kinase 2 alpha', casein kinase 2, alpha prime polypeptide
  • Host
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CSNK2A2 using anti-CSNK2A2 antibody (A04722-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2A2 antigen affinity purified polyclonal antibody (Catalog # A04722-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2A2 at approximately 39 kDa. The expected band size for CSNK2A2 is at 41 kDa.
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